Determination of the Vitamin A, E, D₃ in Powder Milk with HPLC-electrochemical Detector

A HPLC method with the electrochemical detector for the determination of the Vitamin A,E,D₃ in powder milk is studied.The mobile phase is CH₃OH:H₂O=95:5(V/V)with 0.05 mol/L NaClO₄.The parameter of detector is: glassy carbon as working electrodes,1.3 V DC.The detection limit of V_A,V_D3,α-VE,γ-VE,δ-VE is 0.08,2.3,0.16,0.19,0.28 ng respectively and the recovery rate is 90.2%~98.2%,91.2%~103%,92.7%~99.5%,92.6%~97.4%,90.7%~98.0% respectively.This method is characterized by high specificity and sensitivity,compared with the UV detection.