Abstract: The method for determination of 7sweeteners in wine, grape wine and juice has been developed using the ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) with ammonium acetate solution/methanol as the mobile phase. Prior to centrifuge with 13000 r/min for 10 min, samples were diluted. Under optimized conditions, the limits of detection(LOD, S/N=3) were from 0.003 to 8μg/L and the linear range was between 10 and 500μg/L with R² ≥ 0.9981. Recoveries were in the range of 77.10%~118.94%, and RSDs of the peak areas were between 0.19% and 5.24%. This method is simple, sensitive and reliable, and is suitable for the rapid detection of artificial sweeteners in these three drinks.