Abstract: A high performance liquid chromatographic method using vortex mixing assisted ultrasonic extraction has been developed for the simultaneous determination of benzene homologues and phthalic ester plastifiers in water-borne adhesives. In order to disperse the sample matrix into the extraction solvent, samples were distributed and then extracted by the use of a vortex mixer, and an ultrasonic generator. The type of solvent and extraction time on the effect of the target extraction efficiency were discussed. Chromatographic conditions, such as mobile phase, detection wavelength, flow rate, and sample volume, were optimized. Under the optimal conditions, the correlation coefficient (R²) of benzene homologues and phthalic esters were greater than 0.997, the average recoveries of the method were in the range of 91.9%~111.4% and 88.3%~121.4%, respectively, with relative standard deviations of 1.41%~2.80% and 1.24%~2.94%, respectively. The detection limit and quantitation limit of benzene homologues and phthalic esters were 0.3~0.6 mg/kg and 0.2~0.3 mg/kg, respectively. The results showed that,the method is simple, sensitive, and accurate. The analytical results accorded with those obtained by the current standard recommended methods, and is suitable for the simultaneous determination of benzene homologues and phthalic ester plastifiers in water-borne adhesives.