meso-四-4-(Boc-苏氨酸)氨基苯基卟啉荧光探针对L-色氨酸的痕量测定

Determination of Trace Amounts of L-tryptophan Using Tetra-(4-Bocthreonine)-aminophenylPorphyrin as Fluorescence Spectral Probe

  • 摘要: 利用荧光光谱方法探讨了以对称型阳离子氨基酸卟啉:meso-四-4-(Boc-苏氨酸)氨基苯基卟啉(TAPP-Thr-Boc)作为光谱探针测定L-色氨酸的最佳条件. 实验结果表明:双-2-乙基己基硫代琥珀酸钠(AOT)表面活性剂的加入能显著增加体系的灵敏度. 在最佳实验条件下,体系荧光强度的下降程度与L-色氨酸含量在0.10~8.80 μg/mL范围内呈线性关系,检出限为0.034 μg/mL. 方法抗干扰能力强,有较好的选择性和灵敏度,用于实际样品仔猪饲料的测定,结果满意.

     

    Abstract: The optimum experimental conditions for the determination of L-tryptophan were investigated by fluorescence spectrophotometry using tetra-(4-Bocthreonine)-aminophenylporphyrin as the fluorescence spectral probe. Experiments indicated that the sensitivity of the system can be enhanced significantly by the addition of bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT). Under optimum experimental conditions, the decreased of the fluorescence intensity of the system was in proportion to the concentration of L-tryptophan in the concentration range of 0.10~8.80 μg/mL with a limit of detection of 0.034 μg/mL. The method has been used in the determination of samples with satisfactory results.

     

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