Abstract: A qualitative and quantitative analytical method was developed for the determination of buprofezin, fosmet and pyridaben by solid phase extraction on gas chromatography-ion trap mass spectrometry in tea. The separation and quantitative analysis of 3 pesticides was performed by the gas chromatography-ion trap mass spectrometry. The results showed that recoveries of 3 pesticides ranged from 92.0% to 115.0%. The detection limits were in the range of 0.01~0.02 mg/kg. The method is simple, and is of high efficiency and sensitivity for the simultaneous determination of buprofezin, fosmet and pyridaben in tea sample.