阴离子色谱-积分脉冲安培法测定珍稀药材金线莲中游离氨基酸

Determination of Free Amino Acids in Precious Substances of Anoectochilus Roxburghiiby Anion Chromatography-Integral Pulse Ampere

摘要: 目的：定性及定量分析珍稀药材金线莲中的游离氨基酸.方法：将高效阴离子交换色谱-积分脉冲安培检测法应用于珍稀药材金线莲游离氨基酸的分析测定.结果：17种氨基酸的检出限在0.23～3.37 pmol之间，峰面积校正曲线的线性范围为2～3个数量级，5次平行测定的峰面积的相对标准偏差RSD在0.96%～4.2%之间.结论：方法适用于药材金线莲中氨基酸含量的测定，结果良好.干燥金线莲药材的加入回收率分别在88.9%～108.0%之间.

Abstract: Objective:The qualitative and quantitative determination of free amino acids in precious medicinal material Anoectochilus roxburghii.Method: The anion chromatography-integral pulse ampere method is used in the qualitative and quantitative determination of free amino acids in precious medicinal material Anoectochilus roxburghii.Results: The limits of detection (IOD) for 17 amino acids fell between 0.23~3.37 pmol, the linearity range of peak area calibration curve (profile) was the order of 2-3 and RSDs for five determinations were 0.96%~4.2%.Conclusion: This method is applicable to the determination of free amino acids in the medicinal material Anoectochilus roxburghii with good results. And the recovery of dried Anoectochilus roxburghii was 88.9%~108.0%.