Abstract: Based on the determination of contents of active components in Xianglianwan pills and comprised medicinal materiinals, an available and practical method for controlling the quality of preparation and medicinal materials has been set up. The contents of costunolide and dehydrocostuslactone were assayed under chromatographic conditions as follows: Hypersil ODS column (5 μm, 4.6 mm × 250 mm), 30 ℃, mobile phase of acetonitrile and water (70∶30, V/V) at a flow rate of 1.0 mL/min, and the detective wavelength of 210 nm. Jatrorrhizine，palmatine，berberine in Xianglianwan pills and corresponding comprised medicinal materials were simultaneously determined under the following conditions: Kromasil ODS (5 μm, 4.6 mm × 150 mm), the column temperature 30 ℃, phase flow rate of 1.0 mL/min, and detective wavelength, 350 nm.All the compounds showed good linearity (r> 09993) in the range of test concentrations, average recoveries of the method were between 87.67% and 102.37%, RSD＜313%, the results showed that this method is simple, sensitive, accurate and reproducible.