高效液相色谱法测定竹节参中多种人参皂苷含量

Determination of Ginsenosides in Panax Japonicus C.A. Mey by HPLC

摘要: 建立了高效液相色谱法（HPLC）测定竹节参中人参皂苷R_g1、R_e、R_b1、R_b2、R_g2、R_d含量的方法.运用二极管阵列检测器（DAD）峰纯度和光谱检索功能，结合保留时间定性，外标峰面积法定量.采用C₁₈反相柱，以乙腈-水梯度洗脱测定了同一批竹节参总皂苷中人参皂苷R_g1、R_e、R_d的含量分别为0．81%、0．15%、2．99%，回收率为93.46%～94.02%，含量及回收率的RSD均小于5%，该方法简便、灵敏，精密度及准确度在允许范围内，可作为竹节参皂苷提取物中多种人参皂苷的同时测定方法.

Abstract: A HPLC method for determination of ginsenosides including R_g1、R_e、R_b1、R_b2、R_g2、R_d in panax japonicus C.A.Mey was reported. HPLC analysis was performed on a C₁₈ reversed-phase column (5 μm 、4.6 mm×250 mm)using mobile phase of acetonitrile and water mixture under gradient elution.Ginsenosides Rg1、Re、Rd in a bath of chilkusetsusaponin extract were tested .The contents of R_g1、R_e、R_d were 0.81% , 0.15%，2.99% respectively and the recovery was 93.46%~94.02%. Both sides relative standard deriations were less than 5%. This method is simple, sensitivity, reproducibility and can be used for the determination of ginsenosides in panax japonicus C.A.Mey by HPLC.