Abstract: In order to determinate the polyphenols content quickly in cigarette, the near infrared mathematical models of polyphenols in cigarette were founded by the near infrared reflectance spectroscopy and chemometrics. The result showed the average relative error and RSD of the model external verification of the chlorogenic acid, isochlorogenic acid and rutin were within 5% .The models were accurate and stable and could be used to determinate quickly the polyphenols components accurately in cigarette without pollution.