高效液相色谱法测定保健食品中大豆异黄酮含量

Determination of Soybean Isoflavones in Health Foods by HPLC

摘要: 建立了高效液相色谱法（HPLC）测定保健食品中大豆异黄酮含量的方法，运用二极管阵列检测器（PDA）建立了4种大豆异黄酮D、G、De、Ge的光谱库，测定了D、G、Ge与De响应值之比.采用PDA检测，C18反相柱，以甲醇∶水∶醋酸（HAC）=55∶45∶1（V/V/V）为流动相测定了同一批保健食品美龄营养片中4种大豆异黄酮D、G、De、Ge的含量分别为2.24%、10.82%、0. 085%、0.027%，变异系数小于5%，回收率为92.51%～99.30%.该方法快速，精密度及准确度在允许范围内，可作为保健食品中D，G，De，Ge的同时测定方法.

Abstract: A HPLC method for determination of soybean isoflavones including D, G, De, Ge in health foods was reported, The 1-4th derivative spectra of D, G, De, Ge was eatablished by PDA.The sample was analyzed on a C18 reversed-phase column (5 μm, 4.6×250 mm) with a isogradient mobile of methanol∶water∶HAC=55∶45∶1(V/V/V) .ISO in a health Meiling nutritions tablets was tested.The contents of D,G, De, Ge were 2.24% , 10.82%，0.085%，0.0027% respectively, and variation coefficient was less than 5%, and the recovery was 92.51%~99.30%.This method is simple, sensitivity, reproducibility and can be used for the determination of 4 soybean isoflavones in health foods.