高效液相色谱法研究当归指纹图谱

赵健雄; 封士兰; 胡芳弟; 刘欣; 李应东

高效液相色谱法研究当归指纹图谱

Studies on Chromatography Fingerprint(CFP) of Danggui by High-performance Liquid Chromatography

摘要

摘要: 目的: 研究当归药材的指纹图谱. 方法: 高效液相色谱法,Hypersil ODS柱(4.6 mm×250 mm,5μm),Kromasil ODS保护柱(4.6 mm×10 mm,5μm).甲醇-1%醋酸梯度流动相,流速1.0 mL/min,柱温25℃.以当归对照药材为对照品,色谱峰光谱采集范围:190~400 nm;以阿魏酸为参照物并测定了阿魏酸的含量,检测波长:323nm,流动相:甲醇-1%醋酸(40:60,V/V),流速0.7 mL/min. 结果: 找出了22个共有峰,其中5号峰为阿魏酸,11个样品与当归对照药材之间的相似度均在90%以上,平均相似度为96.77%. 结论: 样品处理方法简单,研究所得的当归指纹图谱稳定性、重复性好,可以作为当归极性部分的特征性指纹图谱.

Abstract: Objective: Study on chromatographic fingerprint of Danggui herbs. Method: High-performance liquid chromatography on a Hypersil ODS column with HOAc(1%)-methanol gradient elution,flow-rate of 1.0 mL/min,column temperature of 25℃,standard such as Danggui standard,acquisition wave range was 190～400 nm.Determined content of ferulic acid with PAD.Mobile phase: Methanol-1% HOAc(60:40,V/V),flow-rate of 0.7 mL/min,detector wave: 323 nm. Result: 22 common peaks were pointed out,5th peak was ferulic acid,their similarity of 11 samples were more than 90.0%. Conclusion: Handle method of samples was simple,stable and repeatable.The chromatographic fingerprint of Danggui samples could be used to aqueous solution section of them characterized chromatographic fingerprint.

HTML全文

参考文献(0)

施引文献

资源附件(0)