肿瘤标记物1-甲基鸟苷的微分脉冲伏安法的研究

Differential Pulse Voltammetry Study on 1-Methylguanosine

摘要: 建立一种灵敏的测定修饰核苷1-甲基鸟苷(1-MG)的电化学分析方法.在pH为2.00的BR缓冲液中,用循环伏安法(CV),线性扫描伏安法(LSV),方波伏安法(SWV),微分脉冲伏安法(DPV)等现代电化学技术研究了肿瘤标记物1-甲基鸟苷在玻碳电极(GCE)上的伏安行为.实验结果表明,pH为2.00的BR缓冲液中,1-甲基鸟苷在+1.16V(vs.甘汞)电位处产生一个灵敏的微分脉冲阳极氧化峰,该氧化峰的峰电流值与1-甲基鸟苷的浓度在4.0×10^-7～1.0×10^-5mol/L范围内呈良好的线性关系,最低检测限达(定义为D=2σ/K)6.6×10^-8mol/L.将该法应用于兔血清及尿样中1-甲基鸟苷的测定,回收率在96.0%～102.0%之间,5次测定的相对标准偏差(RSD)均小于3.5%.并初步研究了1-甲基鸟苷的电化学机理.

Abstract: Voltammetric behaviour of 1-Methylguanosine(1-MG) has been investigated at Glassy Carbon Electrode(GCE) by cyclic voltammetry(CV), linear sweep voltammetry(LSV), differential pulse voltammetry(DPV), square ware voltammetry(SWV). In the Britton-Robinson(pH2.0) buffer solution, one well-defined oxidation peak of 1-MG is proportional to the concentration of 1-MG over the range of 4.0×10^-7～1.0×10^-5 mol/L.The detection limit is 6.6×10^-8 mol/L. This method was successfully applied to the analysis of urine samples and serum sample with recoveries 96.0%～102.0%.