Abstract: A new method for determination of trace molybdenum in ethylene glycol by spectrophotometry was proposed. This method is based on molybdenum react sensitivey with salicyl fluorine and cetyl trimethyl ammonium bromide, a cationic surfactant, to form the stable complexes in the strong acid solution.Its λ_max is 524 nm and its apparent ε₅₂₄ is 1.03×10⁵. The feat condition was studied in detail.The recovery of molybdenum in ethylene glycol ranged from 94% to 98%.The proposed method gave a satisfactory result for the determination of molybdenum in ethylene glycol.