Abstract: Solvent extraction and UV absorbing method were adopted to determine 3,4-benzopyrene content in microstalline wax. Dimethyl sulfoxide was used as extraction solvent. Hexadecane as blank solvent was selected for making a series of standard solutions. The standard curve was made by the UV absorbance and the 3,4-benzopyrene.The results show that the good liner relation is between 50 ng/g and 3 000 ng/g and the correlation coefficient is 0.9995. Detection limit is 16 ng/g. This method gives an accurate and precise result with the RSD less than 6.2%. The recovery is between 97.0%～99.4%.