卡那霉素作为手性选择剂的毛细管电泳手性药物分离研究

Enantioseparation of Chiral Drug with High Performance Capillary Electrophoresis Using Kanamycin as Chiral Selector

摘要: 建立了一种以天然易得的卡那霉素为手性添加剂,用毛细管区带电泳法快速分离市售对乙肝有良好治疗效果的药物联苯双脂衍生物的方法,拓宽了毛细管电泳中手性选择剂的范围.通过实验研究了卡那霉素、甲醇含量、pH值、磷酸盐缓冲体系和硼砂缓冲体系对手性分离的影响,以及三种有机溶剂(甲醇、乙睛、异丙醇)添加剂对手性分离的影响.结果表明:在含有3%卡那霉素、30mol/L硼砂缓冲体系(pH=8.0)添加30%异丙醇是最佳的分离条件.

Abstract: A rapid enantioseparation method for racemic compounds by using kanamycin as a new chiral selector was set up. Two racemic drugs, BPME1 and BPME2, were successfully separated by capillary zone electrophoresis under the optimum separation conditions. The pH value of the background electrolyte, the concentration of chiral selector, the concentration both borax and phosphate were optimized. The effects of organic modifiers on chiral drug resolution were investigated. The use of borax and isopropyl alcohol was found to be effective for the chiral separation.