Abstract: The HPLC technique with mono and di sulfonate separation was demonstrated to base quantities of the two fraction of KPS-2. Chromatographic separation phenomena in sandpacks were readily identified. The method is simple,sensitive and microanalytical. With the application of HPLC technique, chromatographic separation coefficients were given, With sodium carbonate added to surfactant system, the extent of KPS-2 chromatographic separation decraases. It is useful for optimizing chemical flooding formula.