Abstract: By reaction of oligopolyamine, epichlorohydrin alternately with λ chloropropyl triethoxyl silane bonded silica phase, and methylated afterwards, a novel silica based strong anion exchanger was prepared. This anion exchanger possesses the advantages of resin based anion exchangers and silica based anion exchangers for its high ion exchange capacity, good column permeability, being durable to much concentrated buffer and wide pH range from pH 1 to 12, and the unspecific interaction of silanol to solutes being eliminated. This strong anion exchanger can be used successfully in the separation of biopolymers. Proteins, peptides and enzymatic mouse brain RNA have being separated.