反相高效液相色谱法半制备分离T(4-MOP)PS<SUB>4</SUB>卟啉的研究

反相高效液相色谱法半制备分离T(4-MOP)PS₄卟啉的研究

摘要: 本文研究了新水溶性5,10,15,20-四(4-甲氧基-3-磺酸苯基)卟啉T(4-MOP)PS₄的反相高效液相色谱(HPLC)分离条件。采用Shimpack PREP-ODS半制备色谱柱,用含有10mmol/L四乙基碘化铵的乙腈-水(体积比25:75)为流动相,流速18mL/min,于418nm波长下检测。T(4-MOP)PS₄与合成中生成的杂质组分完全分离。经此制备的卟啉纯度高,已成功地应用于自来水样中微量钴、锌、铜离子的HPLC测定中。

Abstract: The semi-preparative high performance liquid chromatographic condition of the title compound, a new water soluble prophrin T(4-MOP)PS₄, has been established. Using a Shimpack PREP-ODScolumn (14μm, 20mmi.d×250mm) as the Stationary phase and the solution of acetonitrile-water (25:75, V/V) containing 10mmol/L tetraethylammonium iodide as the mobile phase, flow rate 18mL/min, T (4-MOP) PS₄ was completely separated from impurities and detected at 418nm. The received.T(4-MOP)PS₄ solution has good purity and has been successfully applied to the HPLC determination of trace cobalt(Ⅱ), zinc(Ⅱ) and copper(Ⅱ)in the tap water sample.