Abstract: A high performance liquid chromatography (HPLC) for the determination of 3 acrylamides (acrylamide, N-hydroxymethacrylamide and methacrylamide) in paper-based food contact materials and products has been established. The mobile phase, column temperature, and extraction conditions were optimized, respectively. The samples were shredded and extracted by ultrasonic extraction with ultrapure water. The extract was filtered through a 0.22 μm polytetrafluoroethylene (PTFE) membrane, eluted by gradient with methanol-water as mobile phase, separated by an Agilent 5 TC-C₁₈(2) (250 mm × 4.6 mm, 5 μm) column, determined by a diode array detector, and quantified by the external standard method. The results showed that the acrylamide, N-hydroxymethacrylamide and methacrylamide had good linear relationships in the concentration range of 0.1~5.0 mg/L, the limits of detection were 0.4, 0.5, 0.4 mg/kg, respectively, the average spiked recoveries rate were 86.7%~103.3%, and the relative standard deviations (RSD, n=6) were 2.1%~6.7% under the optimal conditions. The method is simple and accurate, and fills the gap in the detection methods of N-hydroxymethacrylamide and methacrylamide in the standard system.