Abstract: In order to reduce the impact of benzene derivatives on the health of laboratory personnel and the pollution of environmental air, combined with the characteristics of the isocarbon response of a hydrogen flame ionization detector, the relative standard deviation (RSD) of the relative mass correction factor of benzene series compounds with different concentrations was not more than 4.0%, and the RSD of the relative mass correction factors for benzene series compounds at different times was not more than 3.1%, when measured under the same gas chromatograph and under the same conditions. There was no significant differences with the relative mass correction factors estimated using effective carbon number. In addition, using the method of relative mass correction factor and the external standard for the determination of the standard samples, the relative error of the determination results of each component was not more than 4.7%, indicating that the method of relative mass correction factor has a high accuracy. Therefore, combining the relative mass correction factor measured by the test with the method of external standard to quantify the benzene series can ensure the quality of monitoring results and reduce the types of benzene series standard substances.