Abstract:
The chiral membrane of amylose-tris-(3, 5-dimethylphenylcarbamate)-polyethersulfone was prepared by phase conversion method using the amylose-tris-(3, 5-dimethylphenylcarbamate) as the material. The chiral membrane chromatographic separation of propranolol hydrochloride and metoprolol were studied by using the self-made chiral membrane chromatographic device combined with a high performance liquid chromatograph. The effects of injection volume, flow rate and membrane size on the separation of membrane were studied. Under the optimal conditions, the separation factors (
α) and resolution (
Rs) with water as mobile phase were 3.00 and 0.95 for propranolol hydrochloride, 1.65 and 0.46 for metoprolol, respectively. The study opens up a new way for the isolation and analysis of chiral compounds.