Abstract: In the process of embedding samples in epoxy resin, some samples are easy to float in the resin and not easy to be in fixed position, thus affecting the efficiency of ultrathin sections and the observation effect of the samples under transmission electron microscope. It is especially suitable for the rapid and efficient preparation of nanowires, rods and other cross section samples by embedding and positioning the samples easily floating in epoxy resin for many times, and then preparing the cross section samples for transmission electron microscopy through the steps of block modification and section. The method of multiple embedding is simple and practical, and the sample section prepared by ultrathin section is clearer and more complete.