Abstract: The history of development of super-resolution fluorescence confocal microscopy imaging technology is summarized, and the independent innovation of high-resolution fluorescence confocal microscopy imaging technology in China is summarized. Since 2006, the Chinese Academy of Sciences and Peking University have successfully developed several fairly mature super-resolution fluorescence confocal microscopy systems, which has effectively promoted the development of independent innovational instruments in China. Based on the analysis of the evaluation data of the open sharing of large-scale instruments, in this article on the analysis of the open and sharing use of laser confocal microscopes in universities and research institutes are focused.