Abstract: Ultra performance liquid chromatography (UPLC), which is the new hotspots in the field, provides better separation and faster analysis than high performance liquid chromatography (HPLC) with the properties of particle sizes less than 2 μm and ultrahigh pressure systems(> 105 kPa). The ACQUITY Arc System, designed as the bridge between HPLC and UPLC method, is specifically engineered to respond to the needs of analytical scientists and can enable them to efficiently replicate or improve the established HPLC method, or easily transfer to UPLC performance just with the flip of a switch. The components, the usage and daily maintenance of Waters ACQUITY Arc liquid chromatographic system is introduced in detail. The article provides experience and direction for the beginners. The common faults and solutions of using the instrument are summarized, in order to ensure the accuracy and stability of the testing results and to extend the service life of the instrument.