Abstract: Adopting the gas chromatographic technology, a quantitative analysis method for samples containing 2, 4-glutaric ketone, 2-heptanone and cyclohexanone is set up. Qualitative analysis was carried out using a capillary column Rtx®-5 (30 m×0.25 mm×0.25 μm)and a pure standard material as the control. Standard curve method was used to perform quantitative analysis after the optimization of chromatographic conditions. The linearities of each composition were good because all the linear coefficient r were above 0.999 in the concentration range of 0.1 ~ 0.4 mg/mL for 2, 4-glutaric ketone, 0.7 ~3.5 mg/mL for 2-heptanone and 0.9 ~ 4.4 mg/mL for cyclohexanone respectively. The precision of the method was good for the RSD was less than 5%. The spiked recovery of three components were 92.93%, 103. 17% and 102.96%, respectively, all of which ranged from 80% to 120%, conforming to the requirements of quality control. The method realizes the simultaneous determination of 2, 4-glutaric ketone, 2-heptanone and cyclohexanone, and provides a reference for the determination of correlative ketones.