Abstract: Triclosan in wet wipes was determined by ultra-performance liquid chromatography(UPLC). The sample was extracted with methanol, centrifuged, filtrated and detected using 80% MeOH and 20% H₂O as the mobile phase. The flow rate was 0.2 mL/min. Triclosan was separated on a Phenomenex C₁₈ chromatographic column and detected at 280 nm. The correlation coefficient of this method in 0.50~100.00 mg/L was 0.999 1. The determination limit was 0.30 mg/kg with a method quantification limit of 10.0 mg/kg. The RSD was 2.3% and the recovery was 90.50%~93.50%. This method is simple in sample preparation, it has good precision, accuracy and stability.