Determination of Soybean Isoflavones in Health Foods by HPLC

A HPLC method for determination of soybean isoflavones including D, G, De, Ge in health foods was reported, The 1-4th derivative spectra of D, G, De, Ge was eatablished by PDA.The sample was analyzed on a C18 reversed-phase column (5 μm, 4.6×250 mm) with a isogradient mobile of methanol∶water∶HAC=55∶45∶1(V/V/V) .ISO in a health Meiling nutritions tablets was tested.The contents of D,G, De, Ge were 2.24% , 10.82%，0.085%，0.0027% respectively, and variation coefficient was less than 5%, and the recovery was 92.51%~99.30%.This method is simple, sensitivity, reproducibility and can be used for the determination of 4 soybean isoflavones in health foods.