分析测试新成果(160~168)金属吸附剂用于去除血清蛋白及核苷检测

Removal of Serum Protein and Detection of Nucleosides by Metal Adsorbent

  • 摘要: 利用多糖与金属离子复合制备了一种高效的蛋白质吸附剂.海藻酸钠和羧甲基纤维素钠是两种富含羟基和羧基的多糖, 具有较强的金属亲和性.将其用钙离子交联后制备成金属-多糖复合材料, 进一步修饰铜离子, 得到蛋白质吸附剂.吸附剂对富含组氨酸的牛血红蛋白的吸附量可以达到33 g/g, 对少量组氨酸的牛血清白蛋白的吸附量也可以达到9.8 g/g.蛋白质吸附剂对人血血清进行两次吸附后, 可以去除其中98%的蛋白, 能够满足人血血清中核苷类物质的直接色谱进样检测.

     

    Abstract: A highly effective protein adsorbent was prepared using polysaccharide and metal ions. Sodium alginate and sodium carboxymethyl cellulose are two polysaccharides rich in hydroxyl and carboxyl groups, which have strong metal affinity. Both polysaccharides were cross-linked by calcium ions to prepare the metal-polysaccharide composite material, and further modified with copper ions to obtain the protein adsorbent. The adsorption capacity of the adsorbent for histidine-rich bovine hemoglobin reached 33 g/g, and for bovine serum albumin with a small amount of histidine reached 9.8 g/g. 98% of the protein in blood serum could be removed by the adsorbent after adsorption twice. The nucleoside substances in human serum can be analyzed directly by high performance liquid chromatography after the high-abundant protein is removed.

     

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